The Persians were driven out by Alexander the Great in 334 BC. Between 241 and 133 BC, the city was ruled by the Kings of Pergamon. However, in 133 BC, the Pergamons lost control of the city as it was absorbed by the Roman empire.
St. Paul also visited the city during his third missionary journey through AsiaMinor, which was between 53-57 AD, on his way to Lesbos. From thisperiod onwards, Assos shrunk to a small village, as it has remained ever since. Ruins around Assos continue to be excavated.
The pillars from the ancient port lay in the harbor for over a millennia. Eventually they were probably sold.
In the early 1900s an attempt was made to move the contents of the Temple of Athena. Much of the art has been moved to museums like the Louvre. The art found includes pictures both of mythical creatures and heraldic events.